Revealing the bacteria

One possibility of why RBC MIC is high ( usual culprits excluded)
Revealing the bacteria
"Using our method, the Borrelia bacteria in the red blood cells stand out clearly after one to two days at room temperature. We manage to make the bacteria visible without using staining (which can only be used on dead preparations). Keeping the bacteria alive allows us to monitor the bacteria through the microscope.
The researchers add a dilute saline solution to the blood preparation. The blood cell then swells up and most of the blood plasma breaks up and disappears.
"The swollen red blood cells become clearly visible. The images are sharper and clearer than those produced by other methods we know of, and they often show incredible details. We can observe how the bacteria emerge from the blood cells. Not all of the structures seen when the blood cells expel material are classically shaped Borrelia bacteria. Visualization of various Borrelia life stages ca...Afficher la suite

Classic microscopy reveals borrelia bacteria

phys.org

A simple method has been found that tells people who have become seriously ill after a tick bite once and for all whether they have bacteria in their blood.